Microfluidics based Worm Sorter

Koduri V.V.N.R.M Manohar Prasad[1], John Yan[2], Andrew A.Bettiol[2], K.C James Raju[1]
[1]University of Hyderabad, Hyderabad, Andhra Pradesh, India
[2]National University of Singapore, Singapore
Publié en 2013

The nematode Caenorhabditis elegans is a widely adopted model organism for studying various neurobiological processes at the molecular and cellular level in vivo. With a small, flexible, and continuously moving body, the manipulation of C.elegans becomes a challenging task. A lab-on-a-chip (LOC),is a device that integrates one or several laboratory functions on a single chip of only mm to a few square cms in size, is employed for screening of C.elegans. In particular, pressure driven Micro Fluid channels are of our interest, which are used to sort out C.elegans based on GFP profiles by integrating two sets of emitting and detection fibers . To simulate the behavior of fluid in a channel COMSOL Multiphysics® is used, which motivated us to develop a Y-shaped channel, and calculate the optimum numerical pressure variations such that velocity variations in practice is feasible for sorting.

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