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Seeding Distribution in the Channel of a Cell Culture Vessel
Conventional cell culture (CC) techniques are advancing significantly with a trend towards a mass production. While plating density (number of cells per volume of culture medium) plays a critical role for some cell types, the initial distribution of these cells is affected by the actual size and shape of the space in which cells are introduced. Simulation of the cell seeding distribution showed increased plating density due to boundary layer conditions in off-centerline and loop locations. Transit and sedimentation times estimated by simulation correlate well with experiment. Slight volumetric compression of cells ~9% was determined by matching a buoyancy force to experimental sedimentation time. Simulation indicated possibility towards higher yield performance of the cell culture vessel.
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- brcka_poster.pdf - 9.64MB
- brcka_paper.pdf - 2.94MB
- brcka_abstract.pdf - 0.93MB